Okadaic Acid(DSP) ELISA Test Kit Used For Detecting Mussels

           Okadaic Acid ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8°C *. The shelf life is 12 months when the kit is properly stored

Mussels and Other Shellfish

1. Mussels are removed from their shells, washed with deionized water, thoroughly dried and homogenized using a Polytron or equivalent.

2. A 1.0 g portion of the homogenized mussels is then mixed with 6 mL methanol/deionized water (80/20) using aPolytron or equivalent.

3. Centrifuge mixture for 10 minutes at 3000 x g. Collect the supernatant.

4. Add 2 mL methanol/deionized water (80/20) to the mussel tissue residue. Re-centrifuge mixture for 10 minutes. Collect supernatant and add to the first portion.

5. Bring final volume of collected supernatant to 10 mL with methanol/deionized water (80/20). Filter the extract through a 0.45µm syringe filter (Millex HV, Millipore or equivalent).

6. Add 990 µL of 1X Sample Diluent Buffer to a 4 mL glass vial with lined cap. Add 10 µL of the filtered extract to the diluent (1:100 dilution). Vortex.

7. Analyze diluted extracts as samples.

The Okadaic Acid concentration contained in the samples is determined by multiplying the concentration of the diluted extract by a factor of 1000. Highly contaminated samples outside the range of the curve should be diluted further and re- analyzed. Samples with low concentrations of Okadaic Acid or samples which must meet specific regulatory levels may be analyzed at lesser dilutions.